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논문

Aptamer-based Piezoelectric Quartz Crystal Biosensor

기관명, 저널명, ISSN, ISBN 으로 구성된 논문 개요 표입니다.
기관명	NDSL
저널명	中華醫院感染學雜志 = Chinese journal of nosocomiology
ISSN	1005-4529,
ISBN

저자, 소속기관, 출판인, 간행물 번호, 발행연도, 초록, 원문UR, 첨부파일 순으로 구성된 논문저자 및 소속기관 정보표입니다
저자(한글)	QI, Yongzhi,YAO, Chunyan,LUO, Yang,FU, Weiling
저자(영문)
소속기관
소속기관(영문)
출판인
간행물 번호
발행연도	2008-01-01
초록	OBJECTIVE To establish an aptamer-based piezoelectric .sensor assay to detect human immunoglobulin E (IgE) and acquire the parameters about this kind of biosensor. METHODS Anti-IgE aptamers were immobilized on the gold surface of piezoelectric quartz crystal sensor with biotin-avidin method. The reaction between anti-IgE aptamers and IgE was monitored in time on piezoelectric sensor. Different concentrations of IgE were detected and the calibration curve of IgE was drawn. Non-specific response signals of BSA, IgG and IgE were monitored to show the detecting specificity of aptamer-based sensor. RESULTS For the aptamer-based sensor, the lowest limit of detection was 0. 055mg/L and the linear range was 0.1-2. 5mg/L. Non-specific signals of this sensor were less than 5M of specific signals of IgE at the same concentration. CONCLUSIONS The aptamer-based piezoelectric sensor can detect 5.5 ng IgE while the non-specific signals are very low. So this aptamer-based sensor is hopeful to be applied to clinical laboratory diagnosis.
원문URL	http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART51530989
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