| 초록 |
OBJECTIVE To establish a simple and stable animal model with abdominal infection due to drug- resistant bacteria. METHODS Forty healthy rabbit were infected by Klebsiella pneumoniae ATCC700603, and divided into three test groups.. A1 (bacterial dose 8 ×10~9 CFU/kg), A2 (bacterial dose 16×10~9 CFU/kg) and A3 (bacterial dose 24×10~9 CFU/kg). Normal control group B was established at the same time. Vital signs, white blood cell count (WBC), blood and abdominal irrigating solution cultures and strain identification were observed at 12, 24, 48, 72 and 96h after infection. Patho-samples were obtained after being executed. RESULTS The body temperature, WBC, heart rate (HR) and respiratory rate(R) in group A3 were significantly higher than groups A1 and B,after rabbits having being infected 12-24 hours. And it continued for 96 hours (P〈0.01). Abdominal- irrigating solution and blood cultures were all 100.0% positive in every phase during the study time. And the hacteria strain identification results all were K. pneurnoniae ATCC700603. The total death rate of group A3 was 40.0% after 96 hours. Pathology examination showed there were significant inflammation changes in epiploica, bowel, liver and pancreas, etc. CONCLUSIONS Rabbits can be induced severe acute abdominal infection and keep 96h by applying the method of K. pneumoniae ATCC700603 24 ×10~9 CFU/kg, which can meet many experimental design requirements. |
