| 저자(한글) |
임종희,남정석,제정환,이광훈,이학모,이원우,이병희,정지윤,박재학,이영순 |
| 초록 |
Alginase $Alginase^{ #9441;}$ (Arginine esterase) is one of the snake venoms which is mainly consisted of arginine esterase and acts as a thrombus -forming inhibitor/thrombus-lysin. These present studies were performed to investigate of the acute and subacute toxicity of the Alginase $Alginase^{ #9441;}$ in rats. In acute toxicity study, rats were single administered intravenously with dosages of 0.001, 0.01. 0.1, 1 and 10U/kg B.W. and examined the number of death, clinical sign, body weight and pathological change for 7days after administration of Alginase $Alginase^{ #9441;}$ . At maximum dose level (10U/kg B.W.), Alginase $Alginase^{ #9441;}$ induced symptoms of shock with cyanosis and dyspnea. But these symptoms dissappeared after 30~50 minutes and we could not find any other toxic effect in rats. Therefore, $LD_{50}$ Value of Alginase was over 10U/kg B.W. in rats. In four-week intravenous toxicity study of Alginase $Alginase^{ #9441;}$ , rats were administered intravenously seven days per week for 28 days, with dosages of 0, 0.0125, 0.125 and 1.25U/kg B.W./day, respectively. Alginase $Alginase^{ #9441;}$ did not caused any death and showed any clinical signs in rats. No significant Alginase $Alginase^{ #9441;}$ -related changes were found in feed uptake, water consumption, hematology, serum biochemistry, urinalysis, ocular examination, organ weight and histopathological examination. From the results, Alginase $Alginase^{ #9441;}$ seems not to have any toxic effect in rats when it were given daily intravenous injections below the dosage 1.25U/kg B.W./day for four weeks. |
