| 초록 |
The objective of this study is to estimate the effect of adding TES to LEY and FGE freezing extender for the sperm viability, acrosomal morphology and DNA fragmentation from miniature pig sperm, we evaluated sperm characteristics in TFGE, TLE and LEY with various thawing condition ( $37^{ circ}C$ for 20 sec, 45 sec and $75^{ circ}C$ for 5 sec, respectively), and in different concentration of glycerol at 1%, 1.5%, 3%. The sperm viability and normal acrosome intact(NAI) in TFGE (Viability : $60.3{ pm}2.4$ , NAI : $58.6{ pm}2.2%$ ), TLE ( $61.3{ pm}2.4$ , $62.2{ pm}2.2%$ ) extender significantly(p $50.2{ pm}2.4$ , $54.5{ pm}2.2%$ ) extender thawed at $75^{ circ}C$ for 5 sec. According to the results from glycerol concentration, the viability and NAI of miniature pig sperm in 1.5% glycerol TLE ( $66.1{ pm}3.2$ , $66.2{ pm}1.0%$ ) was highest among the experimental groups. In accordance with this, DNA fragmentation rates was the lowest in TLE ( $43.3{ pm}0.5%$ ) while that in LEY ( $63.5{ pm}2.3%$ ) is the highest. Therefore, these results suggest that TLE extender method for freezing- thawing of miniature pig sperm increased the viability after thawing. |
