| 초록 |
Skin anti-wrinkle activities of the stems and leaves of Abeliophyllum distichum Nakai were evaluated by the extracts obtained from various extraction processes such as using hot water at $100^{ circ}C$ , 70% ethanol at $85^{ circ}C$ , and 70% ethanol with ultrasonication at $60^{ circ}C$ The ultrasonicated extract showed 95.62% of the highest cell viability in addition of $0.3mg/m{ ell}$ of the extracts into the normal human fibroblast cell, CCD-986sk. For antioxidant activities, the extracts using ultrasonicated extract showed the highest DPPH free radical scavenging as 80.27%, followed by 75.88% and 62.44% for the extracts using ethanol extract and water extract. The ultrasonicated extract also showed the highest elastase inhibition activity as 25.32%, compared to ethanol extract and water extract based method at 22.01% and 12.88%, respectively. MMP-1 production was most effectively decreased down to $2908.1pg/m{ ell}$ with ultrasonicated extract while $6640.8pg/m{ ell}$ with water extract and $3609.3pg/m{ ell}$ with ethanol extract, in addition of $0.3mg/m{ ell}$ . Collagen production was increased up to $154.7ng/m{ ell}$ in addition of ultrasonicated extract, and followed by $121.4ng/m{ ell}$ and $31.2ng/m{ ell}$ for ethanol extract and water extract, respectively. These results indicate that the ethanol extract should have skin anti-wrinkling activities and can be improved by the ultrasonication process that high energy input elute more amounts of bioactive substances eluting more amounts of bioactive substances from the high energy input of ultrasonication. |
