| 저자(한글) |
장지훈,정호경,고재형,심미옥,우경완,김태묵,이기호,안병관,조현우,조정희,정원석 |
| 초록 |
Background: Sedum takesimense Nakai has been used as folk medicine in Korea. The present study aimed to determine the biological activity of S. takesimense by investigating the anti-inflammatory effects of S. takesimense water extract (SKLC) on the lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Methods and Results: Cytotoxicity of SKLC on RAW 264.7 cells was determinded by performing MTS assay was found to have no cytotoxic effect on RAW 264.7 cells at a concentration range of $62-500{ mu}g/m{ ell}$ . Further, pretreatment of SKLC inhibited lipopolysaccharide-induced nitric oxide (NO) production in a dose-dependent manner. To determined the inhibitory mechanisms of SKLC on inflammatory mediators, we assessed the inducible nitric oxide synthase (iNOS) and cyclooxygnease-2 (COX-2) pathways. The activities of these pathways were decreased in a dose-dependent manner by SKLC. The production of tumor necrosis factor- ${ alpha}$ (TNF- ${ alpha}$ ), interleukin $(IL)-1{ beta}$ , and IL-6 were also reduced. Conclusions: These results suggest that the down regulation of iNOS, COX-2, TNF- ${ alpha}$ , IL- $1{ beta}$ , and IL-6 expression by SKLC are mediated by the down regulation of nuclear factor- ${ kappa}B$ (NF- ${ kappa}B$ ) activity, a transcription factor necessary for pro-inflammatory mediators. This might be the mechanism underlying the anti-inflammatory effects of SKLC. |
