| 초록 |
The Apostichopus japonicus is an important species in some Asia countries including Korea, China and Japan. The purpose of the present study was to investigate the differential gene expression of heat shock protein90 (Hsp90) and ferritin as a biomarker for the thermal stress during water temperature rising in the sea cucumber, A. japonicus. The A. japonicus (1.4 g) was cultured in incubator of separate temperature ( $15^{ circ}C$ , $20^{ circ}C$ , $25^{ circ}C$ and $30^{ circ}C$ ) for each 0, 3, 6, 12, 24, 48 hours. The mRNA expression levels of Hsp90 and ferritin were examined using RT-PCR assay. Results showed that, the expression of Hsp90 mRNA was not significantly changed at $15^{ circ}C$ . The expression of Hsp90 mRNA was significantly increased at high temperature such as $20^{ circ}C$ and $25^{ circ}C$ . Furthermore, Hsp90 mRNA was early increased at $25^{ circ}C$ than $20^{ circ}C$ . The ferritin mRNA was similar expression pattern with Hsp90. But, Hsp90 mRNA was more sensitive than ferritin mRNA at high thermal stress. These results indicate that Hsp90 and ferritin mRNAs were involved in the temperature changes response and may be play an important role in mediating the thermal stress in A. japonicas. |
