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논문

[Application of molecular chaperones to soluble expression of e23sFv/His fusion proteins].

기관명, 저널명, ISSN, ISBN 으로 구성된 논문 개요 표입니다.
기관명	NDSL
저널명	細胞與分子免疫學雜誌 = Chinese journal of cellular and molecular immunology
ISSN	1007-8738,
ISBN

저자, 소속기관, 출판인, 간행물 번호, 발행연도, 초록, 원문UR, 첨부파일 순으로 구성된 논문저자 및 소속기관 정보표입니다
저자(한글)	Chang, Jing,Ouyang, Qing,DU, Xiao,Yang, Angang,Zhao, Jing,Yan, Bo
저자(영문)
소속기관
소속기관(영문)
출판인
간행물 번호
발행연도	2015-01-01
초록	Objective To explore the feasibility of applying molecular chaperones to the soluble expression of e23sFv/His fusion proteins. Methods The molecular chaperone plasmid pGro7 or pKJE7 was transformed into BL21 (DE3) competent cells together with the prokaryotic expression vector harboring His-tagged e23sFv. The soluble expression of e23sFv/His proteins was induced at 16DegreesCelsius. The yield and antigen-binding activity of the soluble products were compared with those of the insoluble products conventionally purified from inclusion bodies. Results Both the overall yield and the purification ratio of soluble e23sFv/His proteins were relatively lower. The binding affinity of the soluble products to immobilized HER2 was not superior to that of the insoluble products from inclusion bodies. Conclusion The molecular chaperone plasmids pGro7 and pKJE7 partially facilitate the soluble expression of e23sFv/His proteins, but both the yield and the purification ratio are still limited.
원문URL	http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73411385
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