| 저자(한글) |
Li, Zhiyuan,Yang, Gang,Huang, Hua,Xiao, Zhenghua,Ren, Xiaomei |
| 저자(영문) |
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| 소속기관 |
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| 소속기관(영문) |
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| 출판인 |
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| 간행물 번호 |
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| 발행연도 |
2015-01-01 |
| 초록 |
Objective To design a biphasic pulsed electrical stimulation (BPES) system for cardiac tissue engineering and investigate the effect of BPES on the cardiomyocyte-like differentiation of adipose-derived stem cells (ADSCs) in vitro. Methods ADSCs were isolated and cultivated from Sprague Dawley rats. Surface protein expression was detected by flow cytometry to identify the cell phenotype. The multi-differentiation potential of ADSCs was verified by adipogenic and osteogenic inducers. The cells of the third passage were randomly divided into 2 groups: BPES group was subjected to BPES 24 hours after the cells were plated in 6-well culture plates, and BPES was set as 2 ms square pulses delivered at 2 Hz, 2 V voltage amplitude, and lasted 2 hours per day, for 3, 4 or 7 days. The culture medium was replaced every 3 days. Control group was cultured with the same condition but without BPES. The cell morphology was observed under an inverted phase-contrast microscope. Cell proliferation was evaluated by the MTT assay. The expression of cardiac-specific homeobox Nkx-2.5 and connexin 43(CX43) was determined by immunofluorescence cytochemistry. Results The flow cytometry proved that the isolated cells were ADSCs. Oil red O staining showed the fat droplets in the cytoplasm of ADSCs after adipogenic induction. alkaline phosphatase and Von Kossa staining showed calcium nodes in the ADSCs after osteogenic induction. No obvious changes were found in the proliferation of ADSCs. The levels of Nkx-2.5 and CX43 proteins were significantly higher in the BPES group than in the control group. Conclusion BPES system we designed for tissue engineering could promote the differentiation of ADSCs into cardiomyocyte-like cells in vitro. |
| 원문URL |
http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73411390 |
| 첨부파일 |
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