| 저자(한글) |
Zhang, Hui,Zhu, Shuyang,Zhang, Cantang,Liu, Wenjing,Zhu, Jiechen |
| 저자(영문) |
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| 소속기관 |
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| 소속기관(영문) |
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| 출판인 |
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| 간행물 번호 |
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| 발행연도 |
2015-01-01 |
| 초록 |
Objective To investigate the expression of miR-199a-5p in airway smooth muscle cells (ASMCs) of rats in normoxia and hypoxia and its role in the regulation of proliferation and the expression of hypoxia inducible factor 1 alpha (HIF-1관) of ASMCs. Methods ASMCs were prepared by means of adherent culture in vitro. After ASMCs were cultured under normoxia and hypoxia conditions for 24 hours, the content of miR-199a-5p was detected by real-time quantitative PCR (qRT-PCR). The mimic or inhibitor of miR-199a-5p were artificially synthesized and transferred into ASMCs in hypoxia via liposomes. The expressions of miR-199a-5p and HIF-1관 mRNA were detected by qRT-PCR. Western blotting and CCK-8 assay were applied to detect the expression levels of HIF-1관 protein and the proliferation of ASMCs, respectively. Results Compared with the normoxia group, hypoxia significantly promoted cell proliferation and increased the levels of HIF-1관 mRNA and protein. The level of miR-199a-5p decreased in the hypoxia group compared with the normoxia group. The proliferation rate of ASMCs under hypoxia conditions was significantly attenuated by transfection of miR-199a-5p mimic, while it was significantly lifted by transfection of miR-199a-5p inhibitor. Compared with control group, the expression of HIF-1관 protein was reduced in the mimic group and raised in the inhibitor group. There was no significant difference in the content of HIF-1관 mRNA among groups under hypoxia conditions. Conclusion miR-199a-5p can inhibit the proliferation of ASMCs and the expression of HIF-1관 protein in vitro under hypoxia conditions. |
| 원문URL |
http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73411387 |
| 첨부파일 |
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