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논문

[In vitro differentiation of human umbilical cord blood mononuclear cells into mature mast cells induced by cytokines].

기관명, 저널명, ISSN, ISBN 으로 구성된 논문 개요 표입니다.
기관명	NDSL
저널명	細胞與分子免疫學雜誌 = Chinese journal of cellular and molecular immunology
ISSN	1007-8738,
ISBN

저자, 소속기관, 출판인, 간행물 번호, 발행연도, 초록, 원문UR, 첨부파일 순으로 구성된 논문저자 및 소속기관 정보표입니다
저자(한글)	Chen, Huifang,He, Ying,Zhang, Lanzhen,Liu, Zhaoyu,Zou, Zehong,Tao, Ailin
저자(영문)
소속기관
소속기관(영문)
출판인
간행물 번호
발행연도	2015-01-01
초록	Objective To isolate and induce human umbilical cord blood mononuclear cells to differentiate into mature mast cells of high purity. Methods Human umbilical cord blood mononuclear cells were differentiated into mature mast cells by the treatment of recombinant human stem cell factor (rhSCF) and recombinant human interleukin 6 (rhIL-6). The cultured cells at different time points were stained with toluidine blue for the detection of anti-FcepsilonRI and the maturity of mast cells was detected by flow cytometry. After the mature mast cells were stimulated with allergen, the levels of histamine and tryptase in the supernatant were determined. Results The cells started to express FcepsilonRI receptor after 2-week treatment of rhSCF and rhIL-6. After 3 weeks, the amount of FcepsilonRI receptor reached its peak accompanied by increased intracellular basophilic granules. The mature mast cells released tryptase and histamine effectively after allergen challenge. Conclusion Human umbilical cord blood mononuclear cells can be differentiated into mature mast cells by the treatment of rhSCF and rhIL-6. The mature mast cells may be used for the study of allergenicity in vitro.
원문URL	http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73411382
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