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[Preparation and application of polyclonal antibody against amelotin polypeptide].
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 細胞與分子免疫學雜誌 = Chinese journal of cellular and molecular immunology |
| ISSN | 1007-8738, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | Zhang, Juanjuan,Ji, Honghai,Liu, Yunxia,Sun, Yan |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2015-01-01 |
| 초록 | Objective To prepare and identify the rabbit anti-mouse polyclonal antibody against amelotin polypeptide. Methods The polypeptide was synthesized based on the bioinformatics analysis of amelotin, and coupled with keyhole limpet hemocyanin (KLH) for immunization. The amelotin polypeptide-KLH was injected into New Zealand rabbits to prepare the polyclonal antibody. ELISA technology was used to detect the titer of the antibody. The specificity of the polyclonal antibody was identified by Western blotting. The expression of amelotin in the submandibular tissue of mice was observed by immunohistochemistry. Results ELISA showed that the titer of amelotin antibody was 1:1 000 000. Western blotting verified that the antibody had a high specificity. Immunohistochemistry indicated that amelotin was highly expressed in odontoprisis full-thick enamel of 3-day-old and 7-day-old mice, and also expressed in duct epithelial cytoplasm of submandibular glands of 7-day-old mice. Conclusion The polyclonal antibody against amelotin polypeptide has been prepared successfully with high titer and high specificity. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73411395 |
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