| 저자(한글) |
Zuo, Yuanling,Yang, Jianfeng,He, Yang,Zhao, Yiming |
| 저자(영문) |
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| 소속기관 |
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| 소속기관(영문) |
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| 출판인 |
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| 간행물 번호 |
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| 발행연도 |
2015-01-01 |
| 초록 |
Objective To prepare and characterize the monoclonal antibody (mAb) against uncoordinated-5 homolog B (UNC5B) and analyze its effect on the migration of melanoma cells. Methods UNC5B gene fragment was cloned into the prokaryotic expression vector pET-32a. The recombinant UNC5B protein was expressed in E.coli BL21 (DE3) and purified by affinity chromatography. BALB/c mice were immunized with the recombinant protein and the hybridoma cell clones stably secreting UNC5B antibody were screened by traditional hybridoma technique. ELISA, Western blotting and flow cytometry were used to characterize the specificity of the antibodies. In addition, the effect of the mAb on melanoma cell migration was analyzed by wound healing assay. Results The recombinant UNC5B protein was expressed and purified. One high-titer antibody 2C9 was obtained. ELISA, Western blotting and flow cytometry all demonstrated that 2C9 antibody specifically recognized the UNC5B protein. Wound healing assay indicated that the UNC5B mAb could promote melanoma cell migration at the presence of netrin-1. Conclusion A UNC5B-specific monoclonal antibody was prepared and proved to have the ability of promoting melanoma cell migration at the presence of netrin-1. |
| 원문URL |
http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73411389 |
| 첨부파일 |
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