| 초록 |
This study was conducted to evaluate the effects of type of culture media (BM, G2, OS, TCM, and MEM) on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri $F_1$ mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. In vitro maturation was highest in BM followed by the order of OS, MEM, TCM and G2 ( $90{ pm}2.8% 88{ pm}3.2% 85{ pm}4.9% 78{ pm}10.2% 64{ pm}7.7%$ , respectively). To note, the G2 group was statistically different compared to other groups (p $87{ pm}7.2% 85{ pm}6.9% 74{ pm}14.0% 71{ pm}13.8% 2{ pm}1.4%$ , respectively). The MEM group was significantly lower compared to other groups (p $73{ pm}11.6% 71{ pm}9.2% 66{ pm}10.4%$ ). Of note, all cells of the TCM and MEM groups were died during embryonic development. The zona hatched rate ( $51{ pm}9.8%$ vs. $50{ pm}9.1%$ vs. $47{ pm}7.2%$ for BM, G2, and OS respectively) and attached rate ( $45{ pm}12.3%$ vs. $38{ pm}16.1%$ vs. $37{ pm}11.5%$ for BM, G2, and OS respectively) were not different amongst groups. No difference was found in total cell numbers ( $74{ pm}13.9$ vs. $64{ pm}9.2$ vs. $76{ pm}6.7$ for BM, G2, and OS respectively), ICM cell numbers ( $20{ pm}1.9$ vs. $14{ pm}1.8$ vs. $15{ pm}2.1$ ), TE cell numbers ( $55{ pm}12.5$ vs. $49{ pm}10.7$ vs. $61{ pm}5.9$ ), % ICM ( $30{ pm}2.8%$ vs. $24{ pm}7.0%$ vs. $22.8{ pm}2.2%$ ) and ICM:TE ratio ( $1:2{ pm}0.5$ vs. $1:3.1{ pm}0.8$ vs. $1:3.1{ pm}0.5$ ) amongst groups. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice. |
