| 초록 |
This study was conducted to evaluate the effects of different volume ( $100{ mu}l$ vs. 2 ml) of microdrop culture on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri $F_1$ mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. Blastulation rate was not different between groups ( $58.4{ pm}2.9%$ vs. $61.2{ pm}4.8%$ ). Zona hatched rate ( $38{ pm}15.4%$ vs. $27{ pm}3.4%$ ) and attached rate ( $55{ pm}13.9%$ vs. $46{ pm}3.9%$ ) did not differ by the volume of culture media. Total cell numbers ( $59.8{ pm}9.7$ vs. $70.3{ pm}8.7$ ), ICM cell numbers ( $15.8{ pm}0.6$ vs. $16.8{ pm}1.5$ ), TE cell numbers ( $44.0{ pm}9.7$ vs. $53.6{ pm}7.3$ ), % ICM ( $26.4{ pm}2.9%$ vs. $23.8{ pm}3.3%$ ) and ICM:TE ratio ( $1:2.8{ pm}0.4$ vs. $1:3.2{ pm}0.6$ ) were not different between groups (i.e., $100{ mu}l$ vs. 2 ml). These results show that the capacity of the culture medium did not effect the cell numbers of B6D2F1 mice blastocysts. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice. |
