| 저자(한글) |
Wu, Fei,Wang, Lin,Guo, Qiaomei,Zhao, Mingna,Gu, Hongchen,Xu, Hong,Lou, Jiatao |
| 초록 |
IFN- gamma; release assays (IGRAs) have been developed as viable alternative diagnostic tools for detecting latent tuberculosis infection (LTBI). A customized homogeneous sandwich luminescent oxygen channeling immunoassay (LOCI) was used to quantify IFN- gamma; levels in IGRAs. Samples were collected from healthy volunteers (n = 40) who were T-Spot-negative and T-Spot-positive patients (n = 32) at rest. Then the amount of IFN- gamma; in the supernatant of IGRAs was measured by LOCI. The results demonstrated a low background, and high sensitivity, specificity, accuracy, and reproducibility, and a short assay time (only 30 min) with LOCI for IFN- gamma;. The recovery range was 81.63-102.06%, the coefficients of variation were below 5%, and the limit of detection was 19.0 mIU/ml. Excellent agreement between LOCI IFN- gamma; and the T-SPOT.TB test was obtained (97.2% agreement, kappa; = 0.94). The LOCI IFN- gamma; concentrations were significantly higher in T-Spot-positive patients than in the healthy group (p |
