| 저자(한글) |
LIN, Xianghong,LI, Tao,ZHU, Mei,LING, Huazhi,CHEN, Xiaoli,XU, Yuanhong |
| 저자(영문) |
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| 소속기관 |
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| 소속기관(영문) |
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| 출판인 |
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| 간행물 번호 |
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| 발행연도 |
2008-01-01 |
| 초록 |
OBJECTIVE To investigate the antibiotical resistance and to understand the phenotype and genotype of metallo-β-lactamase in Chryseobacterium indologenes. METHODS The MIC to 25 C. indologenes strains was detected by agar dilution method. Phenotype of metallo-β-lactamase was detected by three-disc synergy test and modified three dimension test. Polymerase chain reaction (PCR)amplification for both metallo-β-lactamase and integrase gene was conducted for all isolates. Complete coding gene of metallo-β-lactamase and their DNA sequence analysis was conducted. Conjugation experiment was used to study the transmission of metallo-β-lactamase encoding gene. pIs Of β-lactamase was measured by isoelectric focusing assay. RESULTS The antibiotical resistance of C. indologenes to imipenem and meropenem was 88. 0%, respectively. However, gatifloxacin, levofloxacin and rifampin had better antimicrobiotial ability to C. indologenes compared with other antibiotics in MIC Nineteen strains were identified to produce metallo-β-lactamase using three-disc synergy test and modified three dimension test. Twenty strains were detected to have metallo-β-lactamase genotype by PCR amplification using IND-like common prime sets and roar prime sets of complete coding gene. Among them, 9 strains were detected to have blaIND-1 genotype and 10 strains to have blaIND-2 genotype, strain CI-25 was identified to have blaIND-like genotype. The mutation of base sequences and amino acid sequences in 5 strains were found simultaneously. Conjugation experiment showed that metallo-β-lactamase encoding gene couldn't transfer. Nineteen strains had 1 or 2 bands on isoelectric focusing electrophoresis. Strain CI-5 was proved to have blaIND-1, but phenotype of metallo-β-lactamase was negative. CONCLUSIONS C. indologenes has high rate of metallo-~- lactamase producing, and thus it is difficult to treat. The major genotype of metallo-β-lactamase for C. indologenes is blaIND-1 and blaIND-2 in Hefei. The expression of blaIND could exist negative or low level occasionally. |
| 원문URL |
http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART51530880 |
| 첨부파일 |
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