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논문 기본정보
Detecting Clostridium perfringens Alpha-toxin by Piezoelectric Quartz Crystal Biosensor
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 中華醫院感染學雜志 = Chinese journal of nosocomiology |
| ISSN | 1005-4529, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | ZHAO, Yuhui,CHEN, Ming,HUANG, Qing,YAO, Chunyan,YAN, Huihui,KUANG, Hong,FU, Weiling |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2008-01-01 |
| 초록 | OBJECTIVE To search a method for identifying Clostridium perfringens alpha-toxin (CPα) by piezoelectric quartz crystal biosensor. METHODS A specific oligonucleotide probe for CPα gene according to the base pairing principle was designed and immobilized on the surface of quartz crystal with thiol self-assembly technique. Then CPα target gene obtained by PCR was detected by using the piezoelectric quartz crystal biosensor with mass effect. And the influence of different pH values and different ionic strength for detection were explored. RESULTS CPα was successfully detected by the piezoelectric quartz crystal biosensor. And the optimal conditions for detecting CPα were pH7.6 and 0.64mol/L NaCl solution. CONCLUSIONS The CPα could be detected efficiently by piezoelectric quartz crystal biosensor. Such method can provide a new modality for toxin diagnosis in clinic. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART51530980 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | Clostridium perfringens alpha-toxin,Gene,Piezoelectric quartz crystal biosensor,Oligonucleotide probe |