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논문 기본정보
Characterization of alpha;-Gal Epitope in Cells and Tissues from Homozygous alpha;-1,3-Galactosyltransferase Knockout Pigs
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | Reproductive developmental biology = 한국동물번식학회지 |
| ISSN | 1738-2432, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | Hwang, In-Sul,Kwon, Dae-Jin,Kwak, Tae-Uk,Oh, Keon Bong,Ock, Sun-A,Chung, Hak-Jae,Im, Gi-Sun,Hwang, Seongsoo |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2015-01-01 |
| 초록 | To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred ${ alpha}$ -1,3-galactosyltransferase knock-out ( $GalT^{-/-}$ ) pigs. In this study, the somatic cells and tissues from the $GalT^{-/-}$ pigs were characterized by an analysis of the expression of Gal ${ alpha}$ -1,3-Gal ( ${ alpha}-Gal$ ) epitope. Briefly, ear fibroblast cell lines of 19 homozygous $GalT^{-/-}$ pigs were established and cryopreserved. The expression of ${ alpha}-Gal$ epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS-I-B4 lectin. Also, the homozygous ( $GalT^{-/-}$ ) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of ${ alpha}-Gal$ epitope expression. The results showed that the expression of ${ alpha}-Gal$ epitope in $GalT^{-/-}$ cells (0.2 %) were significantly (p $GalT^{-/+}$ ) (9.3 %) and wild type ( $GalT^{+/+}$ ) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of ${ alpha}-Gal$ epitope was detected a partly in $GalT^{-/+}$ cells and mostly in $GalT^{+/+}$ cells, it was almost not detected in the $GalT^{-/-}$ cells. Also, immunostaining results from various tissues of the $GalT^{-/-}$ pig showed that the expression of ${ alpha}-Gal$ epitope was not detectable, whereas various tissues from $GalT^{+/+}$ pig showed a strong expression of ${ alpha}-Gal$ epitope. Our results demonstrated that ${ alpha}-Gal$ epitope expressions from $GalT^{-/-}$ pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=JAKO201507455618865 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | Xenotransplantation, lt,TEX gt,${ alpha}$ lt,/TEX gt,-1,3-Galactosyltransferase, lt,TEX gt,$GalT^{-/-}$ lt,/TEX gt,pigs, lt,TEX gt,${ alpha}-Gal$ lt,/TEX gt,epitope,BS-I-B4 lectin |