| 초록 |
Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell. Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of $TNF-{ alpha}$ , caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway. Results : The Polygalae Radix water extract $25{ mu}g$ , $50{ mu}g$ , $100{ mu}g$ and $200{ mu}g/mL$ had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $25{ mu}g$ , $50{ mu}g$ and $100{ mu}g/mL$ had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $50{ mu}g/mL$ significantly suppressed the increase in $TNF-{ alpha}$ protein expression in PC-12 cell. The Polygalae Radix water extract $25{ mu}g$ and $50{ mu}g/mL$ significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract $25{ mu}g$ , $50{ mu}g$ and $100{ mu}g/mL$ suppressed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract $25{ mu}g$ and $100{ mu}g/mL$ significantly prevented the decrease in Bcl-2 protein expression in PC-12 cell, Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis. |
