| 저자(한글) |
Lee, Kook Lae,Kim, Chan Gyoo,Kim, Byeong Gwan,Chang, Dong Kyung,Lee, Dong Ho,Kim, Joo Sung,Jung, Hyun Chae,Lee, Yeon,Park, Jong Sang,Song, In Sung |
| 초록 |
BACKGROUND/AIMS: Cytokine plays an important role in initiation and continuation of inflammatory bowel disease. However, cytokine protein has some limitation as a therapeutic tool because of low bioavailability, poor pharmacokinetics and chemical instability. Thus, we studied the effect of interleukin 10 (IL-10) gene transfection on murine colon cancer cell line by using non-viral gene carrier. METHODS: Therapeutic gene and plasmid was pCAGGS mouse IL-10 and gene carriers were polyethyleneimine (PEI) and 3beta[L-ornithinamide-carbamoyl] cholesterol (O-chol). After IL-10 gene transfection, we measured the level of IL-10 in supernatant of cultured CT-26 cells. The chemokine cytokine-induced neutrophil chemoattractant (KC) and macrophage inflammatory protein (MIP)-2, which were treated with lipopolysaccharide (LPS) or tumor necrosis factor-alpha (TNF-alpha), were measured after IL-10 gene transfection. RESULTS: The IL-10 values were increased significantly by using PEI, but not by using O-chol. The KC and MIP-2 values were increased when LPS or TNF-alpha were treated. When PEI was used, KC and MIP-2 values increased by LPS or TNF-alpha were decreased. When O-chol was used, the KC values increased by TNF-alpha were decreased but those treated by LPS were not decreased, and the MIP-2 values were not decreased. CONCLUSIONS: After IL-10 gene transfection in colon cancer cell, IL-10 cytokine was efficiently expressed. The increased chemokine values by LPS or TNF-alpha were suppressed by IL-10 gene transfection, but which was not constant because of carrier efficiency. |
