| 저자(한글) |
Lee, Ji Hwan,Go, Donghyun,Kim, Woojin,Lee, Giseog,Bae, Hyojeong,Quan, Fu Shi,Kim, Sun Kwang |
| 초록 |
This study was performed to investigate whether the spinal cholinergic and serotonergic analgesic systems mediate the relieving effect of electroacupuncture (EA) on oxaliplatin-induced neuropathic cold allodynia in rats. The cold allodynia induced by an oxaliplatin injection (6 mg/kg, i.p.) was evaluated by immersing the rat's tail into cold water ( $4^{ circ}C$ ) and measuring the withdrawal latency. EA stimulation (2 Hz, 0.3-ms pulse duration, 0.2~0.3 mA) at the acupoint ST36, GV3, or LI11 all showed a significant anti-allodynic effect, which was stronger at ST36. The analgesic effect of EA at ST36 was blocked by intraperitoneal injection of muscarinic acetylcholine receptor antagonist (atropine, 1 mg/kg), but not by nicotinic (mecamylamine, 2 mg/kg) receptor antagonist. Furthermore, intrathecal administration of $M_2$ (methoctramine, $10{ mu}g$ ) and $M_3$ (4-DAMP, $10{ mu}g$ ) receptor antagonist, but not $M_1$ (pirenzepine, $10{ mu}g$ ) receptor antagonist, blocked the effect. Also, spinal administration of $5-HT_3$ (MDL-72222, $12{ mu}g$ ) receptor antagonist, but not $5-HT_{1A}$ (NAN-190, $15{ mu}g$ ) or $5-HT_{2A}$ (ketanserin, $30{ mu}g$ ) receptor antagonist, prevented the anti-allodynic effect of EA. These results suggest that EA may have a significant analgesic action against oxaliplatin-induced neuropathic pain, which is mediated by spinal cholinergic ( $M_2$ , $M_3$ ) and serotonergic ( $5-HT_3$ ) receptors. |
