| 초록 |
OBJECTIVE To detect CAT gene and analyze the homology of chloramphenicol resistant enterococci for clinical treatment and prevention. METHODS According to CLSI's guideline, antimicrobial susceptibility tests were performed; CAT gene detected was by PCR and sequencing; the genotypes were analyzed by REP-PCR method; the finger printing was analyzed with Cross-Checker and SPSS11. 0. RESULTS The rate of chloramphenicol resistant enterococci was 21. 9%. CAT gene was detected in 30 isolates of chloramphenicol resistant enterococci. The resistant rates of chloramphenicol resistant enterococci to SMZ-TMP, clindamycin, erythromycin, high level gentamicin, high level streptomycin, dalfopristin, ampicillin, and tetracycline were 100. 0%, 100. 0%, 86.7%, 66.7%, 86.7%, 90. 0%, and 93.3%, respectively. Three groups of Enterococcus faecalis had 100% homology respectively. Two groups of Enterococcus faecium had 100% homology. CONCLUSIONS Chloramphenicol resistance enterococci are multidrug resistant. Chloramphenicol resistance is mediated by CAT gene. REP-PCR method is a simple, rapid, with lower cost and uses to detect the homology in enterococci. |
