| 초록 |
OBJECTIVE To establish a simpler method for screening bacterial metallo-β-lactamase (MBL) by comparing Hodge test and double-disk synergy tests (DDSTs), and using 5 inhibitors such as sodium citrate, EDTANa2, EDTAK2, sodium dimercaptopropane sulfonate and mercaptoethanol. METHODS In Hodge test, 5 inhibitors of different concentration were added to the imipenem(IMP) disks, the sizes of the inhibition zones were compared. Disk containing IMP and disk containing a metallo-β-lactamase inhibitor were used in DDSTs. The optimal inhibitors concentration and edge-to-edge distance between the two disks were selected. The performances of the Hodge test and the DDSTs were also compared. RESULTS Among the metallo-β-lactamase inhibitors used in this study, 1 : 15 diluted mercaptoethanol gave the most reproducible and the clearest results when a filter disk containing mereaptoethanol was placed near the IMP disk with the edge distance of 3 mm. CONCLUSIONS Mercaptoethanol-IMP DDSTs are simple, convenient and sensitive methods for screening MBL. |
