| 저자(한글) |
LIU, Xinyue,WEI, Wansheng,LI, Chunfeng,WANG, Cheng,LU, Minghua,YANG, Lan |
| 초록 |
OBJECTIVE The diagnosis of drug allergy is difficult and is usually based on clinical history, skin tests (for some drugs) and, in a few specialized allergy centers, provocation tests. To establish the method for analyzing basophil activation test (BAT) by flow cytometry (FCM) and evaluate its clinical significance in the diagnosis of drug allergy. METHODS The protocol for FCM analysis of basophil degranulation in allergy dustmite by CD_(63), CD_(203c) and CD_(45) combination was established. The clinical significance of activated basophil by FCM was evaluated by comparing with the results of slgE by fluorescence enzyme-linked absorbent assay (FELISA), regarding the skin prick test as the gold standard. RESULTS Pure basophils were got by CD_(45) and CD_(203c) gating, CD_(63) was the best marker for activated basophil; Spearman's correlation coefficients indicated a moderate positive correlation between SIgE class categorized by Unicap class and activated basophil; there was no significant difference between activated basophil by FCM and slgE by FELISA in the diagnosis of allergic reaction, but the former was better than the latter in specificity and positive likelihood ratio. CONCLUSIONS Quantification of activated basophil by CD_(63) expression with FCM is a valuable new and safe method in vitro for diagnosis of immediate type hypersensitization. |
