| 저자(한글) |
CHEN, Zhanguo,TAO, Zhihua,ZHOU, Wu,DAI, Meijie,LIU, Meina,YU, Zhongxin |
| 초록 |
OBJECTIVE To evaluate the accuracy of TaqMan MGB dual-probe detecting YMDD motif mutation of hepatitis B virus in chronic infected patients. METHODS The concentrations of serum HBV DNA were measured by fluorometric real-time PCR. YMDD motif mutations of HBV were detected by TaqMan MGB dual-probe and sequence analysis respectively. Sequence analysis test was regarded as a reference method. RESULTS Among the 246 cases serum samples, all were positive for HBV-DNA by TaqMan MGB dual-probe, while those in 100 healthy people were negative. There were 106 cases with wildtype of YMDD motif mutations, 140 cases with mutants with YIDD and YVDD. It was competely accordant with the results by sequence analysis. The sensitivity of TaqMan MGB dual-probe was 1 ×10~3 copies/ml. CONCLUSIONS TaqMan MGB dual-probe has the superiority of high sensitivity, high specificity, convenience and quickness. It is reliable to monitor YMDD motif mutation for chronic hepatitis B infected patients treated with lamivudine. |
