| 초록 |
Drug-metabolizing activities of Korean native cattle and swine were investigated from viewpoints of the cytochrome P-450's level, their dependent mixed function oxidase activities, the reactive oxygen species formation and cytosolic enzyme acitivities from each liver homogenates. Level of cytochrome P-450 in the liver microsome of Korean native cattle was $0.28{ pm}0.05nmole/mg$ and that in pigs $0.35{ pm}0.03nmole/mg$ . Level of cytochrome $b_5$ of Korean native cattle was $0.24{ pm}0.06nmole/mg$ , and that of pigs $0.2{ pm}0.05nmole/mg$ , showing no difference between two species. NADPH P-450 reductase were higher in Korean native cattle ( $58.3{ pm}5.3nmole/mg/min$ ) than in pigs ( $29.9{ pm}3.8nmole/mg/min$ )(p $96.5{ pm}12.5nmole/mg/min$ ; pigs, $13.6{ pm}2.1nmole/mg/min$ ), N-benzphetamine N-demethylase (cattle, $5.23{ pm}0.82nmole/mg/min$ ; pigs, $0.76{ pm}0.3nmole/mg/min$ ) and aniline hydroxylase (cattle, $0.95{ pm}0.1nmole/mg/min$ ; pigs, $0.33{ pm}0.08nmole/mg/min$ ) were much higher in Korean native cattle than in swine(p $7{ alpha}$ -hydroxylase was higher in swine ( $90.4{ pm}1.2nmole/mg/min$ ) than cattle (cattle, $32.8{ pm}1.2nmole/mg/min$ ). Interestingly, testosterone $16{ alpha}$ -hydroxylase, a marker enzyme for P-450 IIA was not detected in both animal species. These results suggest that Korean native cattle and pigs have high contents of P-450 IA1 and P-450 IIIA. Total sulfhydryl compound (cattle, $10.3{ pm}1.1nmole/mg$ ; Pigs, $14.5{ pm}1.8nmole/mg$ ) and glutathione related enzymes except glutathione reductase (cattle, $38.1{ pm}7.9nmole/mg/min$ ; swine, $22{ pm}3.6nmole/mg/min$ ) showed higher levels in swine than in Korean native cattle. Superoxide dismutase (cattle, $7.64{ pm}0.84nmole/mg/min$ ; pigs, $4.47{ pm}0.94nmole/mg/min$ ) and catalase (cattle, $30.4{ pm}3.7nmole/mg/min$ ; pigs, $17.2{ pm}1.8nmole/mg/min$ ) were remarkably higher in Korean native cattle than in swine (p |
