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논문 기본정보
Synergistic effect of magnetite and gold nanoparticles onto the response of a label-free impedimetric hepatitis B virus DNA biosensor
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | Materials science engineering. C, Materials for biological applications |
| ISSN | 0928-4931, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | Mashhadizadeh, M.H.,Talemi, R.P. |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2016-01-01 |
| 초록 | A magnetite and gold nanoparticle modified carbon paste electrode (CPE) was prepared for the immobilization of a thiol modified Hepatitis B virus (HBV) probe DNA and determination trace amount of target HBV DNA. Indeed, the sensing platform integrated two nanoparticles that had previously been employed individually in the DNA biosensors. The proposed DNA biosensor could measure target HBV DNA virus concentration with a low detection limit of 3.1 (+/-0.1)x10- 13 M, which was greatly lower than the detection limit reported with gold or magnetite nanoparticles alone. The change of interfacial charge transfer resistance (R CT ) was confirmed the hybrid formation between probe and target HBV DNA. The R CT difference (before and after hybridization with the target HBV DNA) was in a linear relationship with the logarithm of complementary oligonucleotide concentrations in the range of 8.3 (+/-0.1)x10 -13 to 6.4 (+/-0.2)x10 -7 M. In addition, the novel methodology for specific DNA sequence detection was highly selective, repeatable, and reproducible. Finally, this work was successfully utilized for the sensitive and label free impedimetric determination of HBV target DNA in the urine and blood plasma samples. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART73790141 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | Carbon paste electrode,,Impedimetric DNA biosensor,,Hepatitis B virus,,Gold nanoparticles,,Magnetic nanoparticles,,Label free |