| 저자(한글) |
LIU, Xiaolan,SONG, Zhijun,CHEN, Yiqiang,WANG, Lizan,KONG, Jinliang,WANG, Hengzhuang,YAN, Ping |
| 초록 |
OBJECTIVE To understand the importance of quorum sensing(QS)system in Pseudomonas aeruginosa(PAE)biofilm infection in lungs,the pathogenic effects of the wild-type P.aeruginosa PAEO1 were compared with QS double mutants PAEO1 lasR rhlR and PAEO1 lasⅠ rhⅡ in vivo.METHODS Rats were challenged intratracheally with alginate embedded PAE strain PAEO1 and the mutants of QS in the concentration of 1×10~9 colony-forming units per milliliter(CFU/ml).Two weeks post intratracheal challenge with P.aeruginosa,parameters were evaluated.RESULTS Two weeks after challenge,significantly milder microscopic and macroscopic lung pathology(P〈0.01)and lower lung bacterial load(P〈0.01)with lower IL-10(P〈0.001,P〈0.01)production in the lungs were found in the lasR rhlR and lasⅠ rhⅡ mutant groups respectively compared to the wild type PAEO1.CONCLUSIONS The pathogenic effects of the lasR rhlR and lasⅠ rhⅡ mutant are obviously milder than that of the wild type PAEO1 in the animal models.The hosts could better clear the PAEO1 lasR rhlR and lasⅠ rhⅡ mutants compared to the PAEO1 wild type,which induced a higher IL-10 response compared to the QS mutants.The functional las and rhl genes of P.aeruginosa play important roles in the establishment and persistence of the chronic infection in lungs. |
