| 초록 |
OBJECTIVE To observe a time course of keratinocyte growth factor (KGF) and surfactant protein A (SPA) changes in rats with Pseudomonas aeruginosa pneumonia and to find out the significances of them. METHODS Specific pathogen-free male Sprague-Dawley rats were used to study. Standard P. aeruginosa strain ATCC 27853 was instilled into airway by the tracheal route to induce model of pneumonia. Samples of lung tissue and BALF were harvested before infection, and 6 h, 9 h and 72 h after infection. Six rats per each point were sacrificed for harvesting samples. Expression of KGF protein in lungs was detected by Western blotting. Western- dot-blot was used to detect SPA expressions in BALF. RESULTS After infected with P. aeruginosa, KGF protein in lungs was markedly increased, reached the peak at 72 h postinfection. KGF protein level at 72 h postinfection was markedly higher than that of before infection (P〈0. 05). SPA in BALF began to decrease till 6 h after infection, followed by a continuous increase, reaching the peak at 72 h postinfection. With the value before infection, the peak value of SPA was higher than the value before infection (P〈0. 05). CONCLUSIONS There are relative deficit of KGF and SPA expression in infected rats at the early stage of pneumonia. The increase in KGF contributes to the change in SPA after infection. The early stage of pneumonia may be the right juncture for KGF/ SPA vicarious therapy. |
