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논문 기본정보
마이크로 혼합기와 반응기로 구성된 DNA 결찰용 바이오칩에 관한 연구
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 大韓機械學會論文集. Transactions of the Korean Society of mechanical engineers. A. A |
| ISSN | 1226-4873, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | 강도형,안유민,황승용 |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2008-01-01 |
| 초록 | In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500 l/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=JAKO200824550465162 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | 마이크로바이오칩,마이크로혼합기,PVP 표면처리,DNA 결찰,Microbiochip,Micromixer,PVP Coating,DNA Ligation |