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논문 기본정보
Effects of Probe Concentrations on LSAW-bisPNA Gene Sensor Detection System
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 中華醫院感染學雜志 = Chinese journal of nosocomiology |
| ISSN | 1005-4529, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | CHEN, Ming,ZHANG, Ye,WANG, Yunxia,DING, Yi,CAO, Liang,FU, Weiling |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2008-01-01 |
| 초록 | OBJECTIVE To investigate the effects of bis-peptide nucleic acid (bisPNA) probe concentrations on hybridization efficiency with leaky surface acoustic wave (LSAW)-bisPNA gene sensor detection system. METHODS Different concentrations of bisPNA probe varied from 0. 001 to 4. 0 μmol/L were respectively immobilized on the surface of LSAW gene sensor. Then it was hybridized with corresponding HPV target sequence. And then the change in phase and equilibrium time of reaction was recorded and analyzed. RESULTS When bisPNA probe concentration varied from 0. 001 to 4.0 μmol/L, the change in phase resulted in hybridization reaction increased firstly and then decreased, and 1. 0 μmol/L was the watershed. And change tendency of equilibrium time was not obvious. CONCLUSIONS Optimal immobilizing probe concentration is 1.0 μmol/L. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART51530986 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | Peptide nucleic acid,Probe,LSAW,Gene sensor,Hybridization |