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장비
장비 및 시설 기본정보
미생물 동정 시스템
장비 개요
| 기관명 | ZEUS |
|---|---|
| 장비번호 | |
| 제작사 | Bio-rad |
| 모델명 | PCR Primus96 plus DGGE Biorad Dcode system |
| 장비사양 | |
| 취득일자 | 2003-08-22 |
| 취득금액 |
보유기관 및 이용정보
| 보유기관명 | 한국과학기술원 |
|---|---|
| 보유기관코드 | |
| 활용범위 | |
| 활용상태 | |
| 표준코드 | B207 |
| 표준분류명 | |
| 시설장비 설명 | Denaturing gradient gel electrophoresis (DGGE) works by applying a small sample of DNA (or RNA) to an electrophoresis gel that contains a denaturing agent. Researchers have found that certain denaturing gels are capable of inducing DNA to melt at various stages. As a result of this melting the DNA spreads through the gel and can be analyzed for single components even those as small as 200-700 base pairs. What is unique about the DGGE technique is that as the DNA is subjected to increasingly extreme denaturing conditions the melted strands fragment completely into single strands. The process of denaturation on a denaturing gel is very sharp: "Rather than partially melting in a continuous zipper-like manner most fragments melt in a step-wise process. Discrete portions or domains of the fragment suddenly become single-stranded within a very narrow range of denaturing conditions" (Helms 1990). This makes it possible to discern differences in DNA sequences or mutations of various genes: sequence differences in fragments of the same length often cause them to partially melt at different positions in the gradient and therefore "stop" at different positions in the gel. By comparing the melting behavior of the polymorphic DNA fragments side-by side on denaturing gradient gels it is possible to detect fragments that have mutations in the first melting domain (Helms 1990). Placing two samples side-by-side on the gel and allowing them to denature together researchers can easily see even the smallest differences in two samples or fragments of DNA. There are a number of disadvantages to this technique: "Chemical gradients such as those used in DGGE are not as reproducible are difficult to establish and often do not completely resolve heteroduplexes" (Westburg 2001). These problems are addressed by TGGE which uses a temperature rather than chemical gradient to denature the sample.extraction + PCR + DGGE + purification을 통한 최종 미생물 군집도 분석 가능. Band 위치에 따른 미생물 identification을 달리하여 분석.환경 미생물 샘플 분석. 토양폐수슬러지 분뇨등 내부에 포함된 박테리아 군집도의 변화 분석. 환경샘플외 다양한 분야의 샘플 내부 포함된 미생물 군집도 변화 분석. 혼합균주의 identification 가능. |
| 장비이미지코드 | http://nfec.ntis.go.kr/storage/images/equip/photo/201407/.thumb/20140708141322618.jpg |
| 장비위치주소 | 대전 유성구 구성동 한국과학기술원 응용공학동 생명화학공학과 응용공학동 3층 3216 |
| NFEC 등록번호 | NFEC-2014-07-189878 |
| 예약방법 | |
| 카타로그 URL | |
| 메뉴얼 URL | |
| 원문 URL | http://www.zeus.go.kr/equip/read?equipId=Z-NTIS-0040850 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| 주제어 (키워드) |