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장비
장비 및 시설 기본정보
정량 중합효소연쇄반응기
장비 개요
| 기관명 | ZEUS |
|---|---|
| 장비번호 | |
| 제작사 | Applied Biosystems |
| 모델명 | 7500 Fast Real Time PCR System |
| 장비사양 | |
| 취득일자 | 2011-04-12 |
| 취득금액 |
보유기관 및 이용정보
| 보유기관명 | 한국생명공학연구원 |
|---|---|
| 보유기관코드 | |
| 활용범위 | |
| 활용상태 | |
| 표준코드 | B201 |
| 표준분류명 | |
| 시설장비 설명 | 분석하고자 하는 시료 내 target 유전자 발현을 정량적으로 분석한다. 유전자에 특이적으로 결합 할 수 있는 형광물질을 이용 온도에 따라 증폭되는 유전자에 정량적으로 결합된 형광물질을 측정하여 각각의 시료내의 target 유전자 발현을 정량적으로 분석한다. 세포내 각각의 유전자의 발현정도가 다름으로 각 세포의 기능 연구 시 유전자 발현정도에 따라 세포 고유한 기능을 연구 할 수 있다. 이용 범위는 특화된 각각의 세포내 유전자 발현 정도를 측정하여 세포기능연구에 이용될 수 있고 또한 의학이나 식품에서 특정병원균의 유전자를 검출에 이용된다. achieved within +/-5℃ seconds Optics ① Detecting device : Cooled CCD ② The detector can be reduce heat noise that give excellent sensitivity comparing with non-cooled CCD. ③ Filter set : Fluorescence detection via a five-position filter wheel ④ That filters alone as a mode of dye discrimination are only effective if you choose dyes with no spectral overlap. Continuous wavelength detection or optimized filter set with a powerful multicomponenting algorithm it makes highly accurate dye discrimination. Excitation light source : Tungsten-halogen lamp Excitation filter : 5 filter sets Emission filter : 5 filter sets System performance ① Real time throughput : Over 3400 sample wells per day with 96-well block configuration and fast thermal cycling protocol. ② End point throughput : Over 3000 sample wells scan with 96-well block configuration (automated mode) ③ Installation specification : Using the TaqMan RNase P 96-well Instrument Verification Plate the Sequence Detection System can be distinguish between 5000 and 10000 template copies with a 99.7% confidence level. Calibrations of Optic ① The system can detect multiplex dyes in same well using optimized five position fluorescence emission filter wheel which is calibrated Pure Dyes. ② The system can normalize samples to account for small variations in pipetting volume using passive Reference dye. ③ The system accurately monitors and processes data from the exponential phase producing highly accurate and reproducible threshold cycle(Ct) values with its precision optics and sophisticated multi-componenting algorithm. Software (1) Sequence Detection Software ① Plate set-up wizards to allow easy experimental design of even complicated multicolor or multiplex assay ② Real-time monitoring of amplication growth curve ③ Auto-baseline and auto-threshold for simplified data analysis ④ Absolute quantification allows quantification of nucleic abid targets using standards curves ⑤ RQ(Relative Quantitation)gene expression analysis software with powerful data viewing capabilites and automated outlier removal for simultaneous analysis of up to ten 96-well plates ⑥ Automated SNP genotype calling capability with intutive graphical output quality-value assignment ⑦ Simple dissociation-curve data collection and viewing to enable the addition of dissociation stages while runs are in progress ⑧ Tool tips for easy identification of sample wells when viewing amplification curve plots ⑨ Automated lamp-life monitoring and instrument diagnostics (2) 5' Nuclease Assay Probe Design Software ① TaqMan Probe and Primer design : Design PCR primer along with the appropriate probes for use in fluorescent 5' nuclease assays using TaqMan PCR kit ② Multiplex PCR : Multiplex PCR primers on up to 15 DNA templates can be designed ③ Standard PCR : Displays primers in pairs and provides the user with the ability to find the optimum primer pair for an existing PCR primer ④ Nested PCR : Automatically calculates the best internal primer pair and display them along with the external primer pairs ⑤ RT-PCR : Automatically recognizes annotated exon-exon junctions translates DNA sequences to RNA by excising the introns and designs primers spanning the exon-exon boundaries |
| 장비이미지코드 | http://nfec.ntis.go.kr/storage/images/equip/photo/201105/.thumb/20110513095145.jpg |
| 장비위치주소 | 충북 청원군 오창읍 양청리 685-1 한국생명공학연구원 오창캠퍼스 본관동 208호 |
| NFEC 등록번호 | NFEC-2011-05-145658 |
| 예약방법 | |
| 카타로그 URL | |
| 메뉴얼 URL | |
| 원문 URL | http://www.zeus.go.kr/equip/read?equipId=Z-NTIS-0029196 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| 주제어 (키워드) |