보유기관명 |
세명대학교 산학협력단 |
보유기관코드 |
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활용범위 |
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활용상태 |
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표준코드 |
A602 |
표준분류명 |
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시설장비 설명 |
nfrared Fluorescence / Upgrade to Quantitative Westerns / Direct infrared fluorescence detection on the Odyssey Infrared Imaging System provides the established standard for Western blot analysis that cant be equaled with chemi\-luminescence and visible fluorescence. Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence cannot. Strong and weak bands on the same blot are accurately detected without the uncertainty and inconvenience of multiple exposures and without spending time in the darkroom. The Odyssey System gives you clear sharp reproducible bands without fuzziness or blowout. Bands hidden by overexposure with chemiluminescence are clear in Odyssey images. The Odyssey System provides a flexible multifunctional platform to accommodate a variety of applications so you get results faster. One example is the In-Cell Western assay an immunocytochemical technique performed with cultured cells in microtiter plates. This assay uses target-specific antibodies to quantify protein levels in fixed cells. Accuracy reproducibility and throughput are all increased by eliminating time-consuming error prone steps such as lysate preparation and gel electrophoresis. In the visible wavelength range used by most fluorescent imaging systems membranes and plastics produce high background due to light scattering and autofluorescence. This high background limits the sensitivity of visible fluores\-cent systems and makes it nearly impossible to detect low-abundance proteins at endogenous levels. At the infrared wavelengths detected by Odyssey both autofluorescence and light scatter are dramatically reduced. The result is the cleanest background highest signal-to-noise ratios and best detection sensitivity available with a fluorescent system. The two infrared fluorescent detection channels of the Odyssey System enable simultaneous two-color target analysis an advantage thats not available with chemiluminescent or radioactive methods. Two-color Western analysis makes normalization easy and eliminates error introduced by stripping and reprobing or by comparison of separate blots. Supe\-rior image clarity and detail make it easier to detect subtle mobility shifts caused by protein modifications such as phosphorylation. Two separate lasers and detectors simultaneously detect both fluorescent signals. The optical system employs diode lasers and solid-state detectors due to their long lifetimes and very low maintenance requirements. Infrared laser excitation outperforms systems that use white light and filter wheels by delivering higher intensity excitation light to the fluorophore. A variety of fluorescent dyes and stains are compatible with the 685 and 785 nm excitation wave -lengths of the Odyssey Systems two diode lasers. Spectral overlap is minimized by the 100 nm separation of the two detection channels and optical filtering assures that each detector measures fluorescence from only one of the infra\-red dyes. Beams from solid-state 685 and 785 nm lasers are focused to form an excitation spot on the scanning surface. A microscope objective focused on the excitation spot collects light from both fluorescing infrared dyes. Light from the microscope objective is passed through a dichroic mirror that splits the light into two fluorescent signals. The fluorescent signals travel through two independent optical paths and are focused on separate silicon avalanche photodiodes and detected. In this example 700 nm fluores\-cence (IkappaB) is shown in red and 800 nm fluorescence (Tubulin) is shown in green. The two colors were imaged simultaneously in a single scan and can be displayed separately or together in a single image. |
장비이미지코드 |
http://nfec.ntis.go.kr/storage/images/equip/photo/201310/.thumb/20131029151817696.jpg |
장비위치주소 |
충북 제천시 신월동 세명대학교 103-13 세명대학교 한방바이오산업임상지원센터 5층 502 |
NFEC 등록번호 |
NFEC-2011-01-131479 |
예약방법 |
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카타로그 URL |
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메뉴얼 URL |
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원문 URL |
http://www.zeus.go.kr/equip/read?equipId=Z-NTIS-0021201 |
첨부파일 |
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