| 시설장비 설명 |
The association of high resolution field emission scanning electron microscopy (FESEM) with a more efficient system of secondary electron (SE) collection and in-lens specimen position provided a great improvement in the specimen’s topographical contrast and in the generation of high-resolution images. In addition images obtained with the use of the high-resolution backscattered electrons (BSE) detector provided a powerful tool for immunocytochemical analysis of biological material. In this work we show the contribution of the FESEM to the detailed description of cytoskeletal structures of the protozoan parasites Herpetomonas megaseliae Trypanosoma brucei and Giardia lamblia. High-resolution images of detergent extracted H. megaseliae and T. brucei showed the profile of the cortical microtubules also known as sub-pellicular microtubules (SPMT) and protein bridges cross-linking them. Also it was possible to visualize fine details of the filaments that form the lattice-like structure of the paraflagellar rod (PF |
