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장비 및 시설 기본정보
형광측정마이크로플레이트리더
장비 개요
| 기관명 | ZEUS |
|---|---|
| 장비번호 | |
| 제작사 | Molecular Devices |
| 모델명 | Gemini EM |
| 장비사양 | |
| 취득일자 | 2012-01-04 |
| 취득금액 |
보유기관 및 이용정보
| 보유기관명 | (재)홍천메디칼허브연구소 |
|---|---|
| 보유기관코드 | |
| 활용범위 | |
| 활용상태 | |
| 표준코드 | B212 |
| 표준분류명 | |
| 시설장비 설명 | 1. The microplate spectrofluorometer from Molecular Devices provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays 2. The microplate spectrofluorometer with dual monochromators allows researchers to try new and novel dyes without having to purchase expensive filter sets 3. The system uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of fluorophores when used in an assay 4. New fluorophores can be tried without additional filter sets 5. If the best wavelengths for a fluorophore of interest are unknown microplate spectrofluorometer can scan both excitation and emission spectra and determine the optimal settings 6. The top- and bottom-reading optical design of the microplate spectrofluorometer allows for measurements of both solution- and cell-based assays 7. With well scanning multiple points within each well can be read providing a high level of sensitivity for cell-based assays 8. The microplate spectrofluorometer is also optimized for chemotaxis membrane migration assays using migration plates 9. The microplate spectrofluorometer system can interface easily with robotic systems using powerful SoftMax Pro software 10. SoftMax Pro microplate data analysis software provides flexibility in experimental design set-up analysis and reporting providing the opportunity to customize assays 11. The microplate reader can supply software validation instrument validation and software version according to GMP and FDA 21 CFR PART 11 COMPLIANCE.1. Microplate spectrofluorometer System 1) Fluorescence photometric performance (1) Measurement modes : Endpoint Kinetic Spectral scanning & Well scanning (2) Microplate type : 6 12 24 48 96 & 384-well microplates (3) Light Source : Xenon Flash Lamp(1 joule/flash) (4) Average lamp lifetime : 1 billion flashes (5) Optics : Fiber optics (6) System validation : Self-calibration with built-in fluorescence calibrators (7) Scanning provided over full range : 1nm increments (8) Dynamic range : 106 in 96-well black plates-auto gain circuity (9) Detector : High QE Multialkali Photocathode PhotoMultiplier Tube(PMT) (10) Wavelength No. for serial measurement : 4 pairs of Ex & Em wavelengths (11) Reading capability : Top-reading & Bottom-reading (12) Wavelength range ① Excitation : 250 - 850nm ② Emission : 250 - 850nm (13) Wavelength selection : Scanning monochromator tunable in 1 nm increments (14) Wavelength bandwidth ① Excitation : 9nm FWHM(full width half maximum) ② Emission : 9nm FWHM(full width half maximum) (15) Wavelength accuracy : < ± 2.0nm across wavelength range (16) Fluorescence sensitivity(signal 3X standard deviation of baseline) ① Microplate(Top read) - 3.0fmol/well FITC 200 μL in 96 wells - 3.0fmol/well FITC 100 μL in 384 wells ② Microplate(Bottom read) - 8.0fmol/well FITC 200 μL in 96 wells - 8.0fmol/well FITC 100 μL in 384 wells 2) Time-Resolved fluorescence photometric performance (1) Wavelength range ① Excitation : 250 - 850nm ② Emission : 250 - 850nm (2) Wavelength selection : Scanning monochromator tunable in 1 nm increments (3) Wavelength bandwidth ① Excitation : 9nm FWHM(full width half maximum) ② Emission : 9nm FWHM(full width half maximum) (4) Wavelength accuracy : < ± 2.0nm (5) Data collection : 50 - 1450 μsecDNA/RNA농도 미생물 실험 Bacterial Toxin 응용실험 Nitric Oxide 응용실험 1) 일반형광물질 FITC Fluorescein isothiocyanate (FITC) 를 항원 항체 반응에 응용하여 미량의 단백질등 원하는 물질을 정량 정성 assay 하는데 사용하는 장비입니다. 2) 최근에는 DNA RAN 에만 특이적으로 반응하는 형광물질을 이용하여 정량 정성 assay 에도 이용이 가능합니다. 3) 다양한 실험을 위하여 6-well ~ 384-well의 모든 microplate type에 상관없이 reading이 가능한 장비입니다. |
| 장비이미지코드 | http://nfec.ntis.go.kr/storage/images/equip/photo/201203/.thumb/20120324173619.JPG |
| 장비위치주소 | 강원 홍천군 홍천읍 연봉리 101번지 (재)홍천메디칼허브연구소 홍천메디칼허브연구소 2층 효능독성평가실 |
| NFEC 등록번호 | NFEC-2012-03-157683 |
| 예약방법 | |
| 카타로그 URL | |
| 메뉴얼 URL | |
| 원문 URL | http://www.zeus.go.kr/equip/read?equipId=Z-NTIS-0031709 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| 주제어 (키워드) |