냉동조직절편기
기관명 | ZEUS |
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장비번호 | |
제작사 | Thermo Scientific |
모델명 | Cryotome FE |
장비사양 | |
취득일자 | 2008-03-20 |
취득금액 |
보유기관명 | 경기과학기술진흥원 |
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보유기관코드 | |
활용범위 | |
활용상태 | |
표준코드 | B107 |
표준분류명 | |
시설장비 설명 | 1. Cryochamber 1) Chamber Temperature : -35℃ 2) Cryobar Temperature : -40℃ 3) Specimen Demount : +2℃for 30 seconde 2. Microtome 1) Retraction : 40㎛ 2) Total Advance : 25mm or more 3) Cutting Stroke (Max.) : 57mm or more 4) Section Thickness : 1-20 ㎛/1㎛ step 20~60㎛/5㎛ step 3. Control Panel 1) Remaining Specimen Travel Indication : By LCD display 2) Microtome Control : · Touch screen micron selector · Dual speed coarse advance control · Fine rewind control for knife-to-specimen alignment · Rapid reset button to return microtome to the beginning of the advance mechanism Frozen Sections Advantages 1. Speed 2. Preserves many things lost in wax a. Lipids b. Enzymes c. Soluble and diffusable materials d. Antigens for immunofluorescence 3. Less shrinkage Disadvantages 1. Poorer quality 2. Storage of blocks is difficult. 3. Ice crystal damage Freezing techniques 1. Liquid nitrogen (-190O C) 2. Isopentane cooled by liquid nitrogen (-150O C) 3. Solid CO2 (-70O C) 4. CO2 gas (-70O C) 5. Aerosol sprays (-50O C) 6. Pelcool (variable) 7. Freezer compartment (-20O C) Sectioning 1. Cryostat. Cold specimen cold knife cold atmosphere Commonest technique. Best cut unfixed rapid later fixation is possible. Sections adhere well Poor for adipose tissue Equipment can be expensive 2. Freezing microtome technique. Cold specimen (cold knife) room temperature Uses fixed material Relatively thick Floating through Damage Better for lipids and nervous tissues Can cut very large blocks Impregnation Techniques Some tissues may require some support (eg lung) or surrounding medium (eg curettings bone marrow fragments) Suitable media include gelatine (absorbs many dyes) agar (metachromatic) methyl cellulose (‘polycell’) soaps and detergents. Freeze drying 1. Quench (rapid freezing) 2. Sublimation of water at <133 mPa and –40OC) 3. Fi |
장비이미지코드 | http://nfec.ntis.go.kr/storage/images/equip/photo/201108/.thumb/20110829170026.jpg |
장비위치주소 | 경기 수원시 영통구 이의동 864-1번지 광교테크노밸리 경기바이오센터 1층 시료전처리실 |
NFEC 등록번호 | NFEC-2010-12-130134 |
예약방법 | |
카타로그 URL | |
메뉴얼 URL | |
원문 URL | http://www.zeus.go.kr/equip/read?equipId=Z-NTIS-0020720 |
첨부파일 |
과학기술표준분류 | |
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ICT 기술분류 | |
주제어 (키워드) |