고속액체크로마토그래피
기관명 | ZEUS |
---|---|
장비번호 | |
제작사 | Waters |
모델명 | Delta Prep 4000 |
장비사양 | |
취득일자 | 2006-12-11 |
취득금액 |
보유기관명 | 건국대학교 글로컬캠퍼스 산학협력단 |
---|---|
보유기관코드 | |
활용범위 | |
활용상태 | |
표준코드 | B402 |
표준분류명 | |
시설장비 설명 | HPLC Operation A simple way to understand how we achieve the separation of the compounds contained in a sample is to view the diagram in Figure G. Mobile phase enters the column from the left passes through the particle bed and exits at the right. Flow direction is represented by green arrows. First consider the top image; it represents the column at time zero [the moment of injection] when the sample enters the column and begins to form a band. The sample shown here a mixture of yellow red and blue dyes appears at the inlet of the column as a single black band. [In reality this sample could be anything that can be dissolved in a solvent; typically the compounds would be colorless and the column wall opaque so we would need a detector to see the separated compounds as they elute.] After a few minutes [lower image] during which mobile phase flows continuously and steadily past the packing material particles we can see that the individual dyes have moved in separate bands at different speeds. This is because there is a competition between the mobile phase and the stationary phase for attracting each of the dyes or analytes. Notice that the yellow dye band moves the fastest and is about to exit the column. The yellow dye likes [is attracted to] the mobile phase more than the other dyes. Therefore it moves at a faster speed closer to that of the mobile phase. The blue dye band likes the packing material more than the mobile phase. Its stronger attraction to the particles causes it to move significantly slower. In other words it is the most retained compound in this sample mixture. The red dye band has an intermediate attraction for the mobile phase and therefore moves at an intermediate speed through the column. Since each dye band moves at different speed we are able to separate it chromatographically.1. Reservoir : 용매를 저장할 수 있는 용기 2. 용매 펌프 : 일정한 유속과 압력으로 용매를 밀어주는 장치 3. 주입기 : 분석하고자하는 시료를 이동상의 흐름에 실어주는 역할 4. 컬럼 : 충진제가 채워져 있어 실질적인 분리가 이루어지는 곳 5. 검출기 : 분리되어진 물질이 검출되어지는 곳 6. Data system : Detector의 signal을 크로마토그램으로 변화시켜줌식품 의약품 화장품 등 다양한 제품의 성분분석과 그 성분의 정성 정량분석을 할 수 있으며 식품첨가물 당류 아미노산 유해물질의 분석을 하여 제품의 안전성검사에 많이 사용되고 천연물 등의 분리 정제에도 활용할 수 있음. |
장비이미지코드 | http://nfec.ntis.go.kr/storage/images/equip/photo/201310/.thumb/2013102919370632.jpg |
장비위치주소 | 충청북도 충주시 충원대로 268 (단월동) 바이오식의약연구센터 상허연구동 6층 620 |
NFEC 등록번호 | NFEC-2010-12-129037 |
예약방법 | |
카타로그 URL | |
메뉴얼 URL | |
원문 URL | http://www.zeus.go.kr/equip/read?equipId=Z-NTIS-0020259 |
첨부파일 |
과학기술표준분류 | |
---|---|
ICT 기술분류 | |
주제어 (키워드) |